Releasing the vacuum inside vial
Hygetropin® vials are air-vacuumed to preserve the powder. Before using an Hygetropin® hGH vial you should let all air out of the vial, this should be done only once for every new vial to be used. It can be done by inserting a bare needle or one attached to a syringe into the vial to let all air get out of the vial.
Hygetropin® should be used with sterile bacteriostatic water for injection. The water acts as a solvent to dissolve the powder inside the vial and turn it into an injectable solution. Use a new sterile syringe (pin width should be 19G-27G) to pull solvent into syringe (1ml of water or less) Inject the water very slowly in a controlled manner to the side of the vial and let the water slide down the bottom of the vial. Do not push the water directly onto the GH powder! Doing so will damage it. Gently dissolve the drug with a slow, swirling motion. Do not shake vigorously, or the active ingredient might denature. Inspect to see if there is still powder at the bottom of the vial, you may need to let the vial in room temperature for a few minutes in order for powder to dissolve completely.
Using an sterile syringe gently withdraw from the vial the desired dose to be injected and release any unwanted air from the syringe. Hygetropin® should be injected subcutaneously by preferred areas are stomach or upper thighs. Wash hands and rub skin with alcohol pad, with one hand pinch 2" inch fold of skin between your thumb and index finger. Hold the syringe with the other hand. Insert the needle at 45- 90 degrees angle to the pinched skin. Gently pull back the plunger to check for blood. If blood enters the solution do not inject, withdraw the needle and start again at a new injection spot. Push the plunger gently to inject, when plunger is all the way down, remove the syringe gently and lightly hold an alcohol pad on the injection spot. Do not rub the area. Dispose of syringe, needles and alchohol pads are always use new ones for each injection. The injection spot should be varied to prevent lipoathrophy.